Journal: Cancers

Article Title: Multiparametric Characterization of the DSL-6A/C1 Pancreatic Cancer Model in Rats

doi: 10.3390/cancers16081535

Figure Lengend Snippet: List of antibodies used for the immunological analysis of the DSL-6A/C1 tumors.

Article Snippet: CD11a , PE , REA596 , Human cell line , Miltenyi , 130-109-171.

Techniques:

Journal: Channels

Article Title: Cone dystrophy and ectopic synaptogenesis in a Cacna1f loss of function model of congenital stationary night blindness (CSNB2A)

doi: 10.1080/19336950.2017.1401688

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: PKCα , Mouse , Purified Mouse Monoclonal IgG2a , 1:1000 , Cedarlane Labs, Burlington, ON , purified bovine brain PKCα (Clone MC5) , [ ] .

Techniques: Affinity Purification, CRAfT Assay, Purification, Recombinant

Journal: Science Advances

Article Title: The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration

doi: 10.1126/sciadv.abl5942

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: APC anti-human CD11a , Mouse IgG1 , Miltenyi Biotec 130-127-294 , 1:50.

Techniques: Cytometry

Journal: Cancer Cell

Article Title: Multimodal stimulation screens reveal unique and shared genes limiting T cell fitness

doi: 10.1016/j.ccell.2024.02.016

Figure Lengend Snippet: Loss of Icam1 -mediated homotypic T cell interactions amplifies CD8 T cell expansion and improves effector functions shortly after TCR stimulation (A) Overlapping genes from top 50 hits from each screen, genes are ranked by average effect size. (B) STRING protein-protein interaction analysis of shared targets from the two boxes in (A) (32 genes). Interactions include direct (physical) and indirect (functional) associations. (C) Kaplan-Meier OS curves of patients receiving TIL therapy , (Besser cohort) with high or low ICAM1 expression in TIL products. Significance calculated with regular log rank test. (D) Microscopy images of indicated T cells 24 h after CD3 stimulation (n = 7 biological replicates). (E) Viable Ctrl and Icam1 -KO T cell counts under indicated stimulation conditions as in the screens, analyzed with Mann-Whitney test (n = 4–7 biological replicates). (F) Viable B16.OVA cells after 4 days co-culture with indicated T cells, analyzed with Mann-Whitney test (n = 4 biological replicates). (G) Transcriptomic profiling heatmap of indicated T cells with or without 24 h-CD3 stimulation, showing significantly (p value <0.001) differentially expressed genes ( Table S3 ). (H) Proteomic STRING enrichment analysis of differentially expressed proteins comparing Icam1 -KO with Ctrl T cells after 24 h-CD3 stimulation, showing top enriched GO biological process (ranked by enrichment strength (Log 10 (observed/expected)) with FDR < 0.1 ( Table S3 ). (I) Flow cytometry-based cytokine bead array showing cytokines released in the culture medium of indicated T cells after 24 h-CD3 stimulation, analyzed with Mann-Whitney test (n = 7 biological replicates). (J) Viable cell counts of Ctrl or Icam1 -KO T cells ectopically expressing wild type (wt) or mutated ICAM1 (lacking the intracellular domain, dcyt). Cell count was assessed 4 days after CD3 stimulation; analyzed with one-way ANOVA with Holm-Sidak’s multiple comparisons test (n = 3 biological replicates). OE: overexpression. (K) Viable cell counts of Ctrl or ICAM1/2/3 -KO human T cells 1 week after 24 h-CD3 stimulation, analyzed with Mann-Whitney test (n = 5 biological replicates). (L) Viable cell counts of human CD8 cells 1week after CD3 stimulation with or without CD54 (ICAM1) or CD11a (LFA1) blocking antibodies, analyzed with Kruskal-Wallis test with Dunn’s post-hoc test (n = 3 biological replicates). Error bars indicate SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Article Snippet: T cell reactivation was performed in the presence of CD11a antibody (1 μg/ml, clone R7-1, BioXCell), CD54 (1 μg/ml, clone R6-5-D6, BioXCell) antibody, CD178/FasL (10 μg/ml, Clone MFL3 (RUO), BD Pharmingen) or respective isotype controls (1 μg/ml, mouse IgG2a, clone C1.18.4 and mouse IgG1, clone MOPC-21, both BioXCell; 10 μg/ml, Armenian Hamster IgG1, κ, BD Pharmingen).

Techniques: Functional Assay, Expressing, Microscopy, MANN-WHITNEY, Co-Culture Assay, Flow Cytometry, Cell Counting, Over Expression, Blocking Assay

Journal: Cancer Cell

Article Title: Multimodal stimulation screens reveal unique and shared genes limiting T cell fitness

doi: 10.1016/j.ccell.2024.02.016

Figure Lengend Snippet:

Article Snippet: T cell reactivation was performed in the presence of CD11a antibody (1 μg/ml, clone R7-1, BioXCell), CD54 (1 μg/ml, clone R6-5-D6, BioXCell) antibody, CD178/FasL (10 μg/ml, Clone MFL3 (RUO), BD Pharmingen) or respective isotype controls (1 μg/ml, mouse IgG2a, clone C1.18.4 and mouse IgG1, clone MOPC-21, both BioXCell; 10 μg/ml, Armenian Hamster IgG1, κ, BD Pharmingen).

Techniques: Purification, Blocking Assay, Control, Functional Assay, Recombinant, Staining, Cell Culture, Isolation, Binding Assay, CRISPR, Sequencing, RNA Sequencing, Software

Journal: Immunity

Article Title: Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function

doi: 10.1016/j.immuni.2020.07.006

Figure Lengend Snippet:

Article Snippet: Mouse anti-CD11a PE-Cy7 Clone HI111 , BD Biosciences , Cat No. 561387; RRID: AB_10611572.

Techniques: Recombinant, Molecular Cloning, Blocking Assay, Conjugation Assay, Staining, Western Blot, Lysis, Mutagenesis, Isolation, Luminex, Transfection, Amplification, Software, Variant Assay